However,
MSCs can lose their differentiation capacity over time in this culture system.
In addition, the available surface area for the propagation of cells is
limited. Therefore, various three-dimensional (3D) culture systems have been
developed. In this study, we developed the scaffold-free 3D culture systems for
the expansion of periosteum-derived progenitor cells (PDPCs) as spheres. The
spheroid formation of PDPCs was induced using a rotation platform. The spheres
maintained their viability and proliferation ability. Moreover, expression
levels of the stemness marker genes and proteins were higher in cells grown on
3D culture system than in 2D culture system. In conclusion, a simple and
economical 3D culture system has been developed that can increase the potential
of PDPCs for clinical use.
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