Enzyme assay for pyridoxal 5′-phosphate by apo-d-amino acid aminotransferase
d-AAT
from thermophilic Bacillus sp. YM-1 was inactivated by ultraviolet irradiation.
The activity was restored by the addition of PLP and showed a linear
correlation with respect to PLP concentrations in the range of 10-40 nM.
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